bta-mir-185: Bta-mir-185 is a microRNA that has been studied in the context of milk exosomes under normal conditions and after S. aureus infection. It has been found that bta-mir-185 remained constant in terms of expression between day 9 and day 16 [PMC5487392]. Functional validation has shown that bta-mir-185 targets the PGM1 gene [PMC6896338]. Target prediction programs have identified a total of 814 target genes for bta-mir-185 [PMC6896338]. The binding sites for bta-mir-185 in the 3'-UTR of target genes have been analyzed using bioinformatics methods [PMC6896338]. Luciferase assays have confirmed that DYRK1B, MLLT3, HP1BP3, NPR2, and PGM1 are target genes of bta-mir-185 [PMC6896338]. Bta-mir-185 has been found to be highly expressed in milk exosomes from cows infected with S. aureus compared to control cows [PMC6896338]. It has also been identified as targeting MLLT3, DYRK1B, and NPR2 genes [PMC8578396]. Bta-mir-185 is evolutionarily close between cows and wild yaks compared to other microRNAs such as bta-miR-24-3p and bta-miR-874 [PMC8578396]. In milk from mammary glands infected with S. aureus, bta-mir-185 is significantly up-regulated along with miR-378 and miR146b [PMC7401532]. References: [PMC5487392]: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5487392/ [PMC6896338]: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6896338/ [PMC8578396]: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8578396/ [PMC7401532]: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7401532/