rno-mir-185: Rno-mir-185 is a microRNA that has been studied in various contexts. In a study on polycystic ovary syndrome (PCOS) in rats, the rats were divided into different groups, including a PCOS + rno-mir-185 group, where the rats were infected with rno-mir-185 overexpressing lentivirus [PMC7373746]. Lentivirus overexpressing rno-mir-185 was injected into the ovaries of PCOS rats [PMC7373746]. In another study on ischemia-reperfusion (IR) injury, rno-mir-185 was found to be one of the miRNA-hubs observed at the late phase post-IR injury [PMC4424502]. It was also found that rno-mir-185 participates in four different loop motifs in the late phase of post-IR injury, which are linked to inflammatory responses [PMC4424502]. To analyze the expression of rno-mir-185, quantitative PCR was performed using specific primers and SYBR Green Master Mix [PMC8093826]. Overall, these studies highlight the involvement and potential role of rno-mir-185 in PCOS and IR injury.