mmu-mir-34a: Mmu-mir-34a is a type of microRNA that has been studied in various contexts. It has been found that the expression of mmu-mir-34a increases in the brain of older rodents, and a decline in its expression is associated with longevity in calorie-restricted mouse brain [PMC3229974]. The miR-34 family, including mmu-mir-34a, has been found to be up-regulated after exposure to ENU and maintained increased expression at subsequent time points [PMC3091750]. Additionally, microarray results have shown increased expression of mmu-mir-34a in apoE−/− and B6 aortic tissue [PMC3641397]. The overexpression plasmid for mmu-mir-34a was constructed using the pEM157 vector [PMC5220162]. Furthermore, a recombinant adenovirus was generated to overexpress miR-34a using the pacAd5 miR-GFP/Puro Shuttle Vector [PMC5220162]. It is worth noting that there is high sequence similarity between miR-34a/34b-5p sequences, allowing for targeting of both mmu-mir-34a and mmu-miR-34b-5p with anti-miRNA molecules [PMC2577349]. Finally, it has been observed that there are five eQTLs on different chromosomes associated with variation in mmu-mir-34a expression levels [PMC3130556].