cfa-mir-34a: Cfa-mir-34a is a microRNA that has been found to be differentially expressed in different groups of dogs with liver shunts. It has been shown to have a high diagnostic performance in differentiating splenocaval shunts from healthy controls, with an AUC of 0.96 (95% CI: 0.86-1.0, p < 0.0001) [PMC7356535]. Additionally, cfa-mir-34a has been found to be significantly expressed in dogs with splenocaval, RGC, RGC-CL, right divisional, and left divisional shunts compared to controls [PMC7356535]. It is part of the miR-34 family and is transcribed from regions of chromosome 5 in dogs [PMC4049822]. Other miRNA families that have been found to be significantly up-regulated with testicular RA intervention include miR-200, Mirlet-7, miR-125, miR-146, miR-23, cfa-miR-184, cfa-miR-214 and cfa-miR141 [PMC4049822]. These findings suggest that cfa-mir34a and other miRNAs could serve as non-invasive biomarkers for liver shunt diagnosis and could reflect the histopathological and molecular events occurring in the liver [PMC7356535].