mtr-miR396a-5p: mtr-mir396a-5p is a microRNA that has been studied in relation to salt stress and drought stress in plants. In a study, it was found that over-expression of miR396a down-regulated GRF1 and further down-regulated WRKY28, suggesting that the miR396a/GRF1/WRKY28 module enhances tolerance to salt stress by promoting plant growth [PMC9011107]. Another study found that miR396a targeted TGA2.3, indicating that the miR396a/TGA2.3 module enhances salt tolerance by mediating ion homeostasis [PMC9011107]. Additionally, mtr-mir396a-5p was found to be downregulated in response to drought stress [PMC8369265]. In another experiment, the expression of mtr-mir396a-5p was significantly downregulated in comparison to control conditions [PMC8369265]. To validate the expression of miRNA and its target genes, qRT-PCR validation was performed on mtr-mir396a-5p and other selected miRNAs [PMC7016817]. Furthermore, mtr-mir396a-5p was identified as one of the most enriched miRNAs in response to drought stress [PMC7016817]. Overall, these studies highlight the role of mtr-mir396a-5p in enhancing plant tolerance to salt stress and its involvement in response to drought stress.