stu-miR396-5p: Stu-mir396-5p is a type of microRNA that shows a significant decrease in expression from the seedling stage to the budding stage in potato plants, particularly in the Yanshu4 variety, when subjected to different levels of nitrogen (N) application [PMC9547441]. There is a potential binding site between stu-mir396-5p and StNiR, and a splicing relationship between the 8th base of stu-mir396-5p and the 8th base of StNiR from the 5' end of stu-mir396-5p [PMC9547441]. This suggests a negative correlation between stu-mir396-5p and StNiR under N stress conditions [PMC9547441]. In addition, under salt stress conditions, stu-mir396-5p is down-regulated in both roots and leaves of "Xushu 22" potato plants, as revealed by qRT-PCR analysis [PMC9302446]. Degradome sequencing further identified that stu-mir396-5p targets growth-regulating factor (GRF) family members such as GRF2, GRF3, and GRF6 [PMC9302446]. Furthermore, differential expression analysis showed that LN_YLS had the largest proportion of differences in stu-mir396-5p expression under treatment conditions [PMC9547441]. In summary, stu-mir396-5p is a microRNA that exhibits decreased expression during different developmental stages and under various stress conditions in potato plants. It shows potential interactions with StNiR and targets GRF family members. The differential expression analysis suggests significant variations in its expression levels depending on nitrogen application levels.