cfa-mir-205: The study conducted qPCR to validate the expression levels of four randomly selected DE-miRNAs, including cfa-miR-30a, cfa-miR-124, cfa-mir-205, and cfa-miR-222 [PMC4768678]. It was found that MMD was upregulated while cfa-mir-205 was downregulated in the CFDP1_vs_CFDP2 condition [PMC4768678]. The study suggested that cfa-mir-205 might play a crucial role in upregulating MMD and enhancing the immune response in the pituitary and hippocampus under chronic stress exposure [PMC4768678]. The analysis of mRNA-seq and miRNA-seq studies identified a potential role for cfa-mir-205 in regulating MMD in the pituitary under chronic stress exposure [PMC4768678]. The dual luciferase reporter assay further confirmed a significant target relationship between cfa-mir-205 and MMD [PMC4768678]. qPCR analysis of brain tissues showed that both MMD and cfa-mir-205 expression levels were examined in the CFD brain, cerebellum, hippocampus, and hypothalamus tissues [PMC4768678]. The study suggested that cfa-mir-205 might play an important role in regulating MMD under chronic stress exposure based on its expression levels [PMC4768678]. It was hypothesized that MMD would be upregulated while cfa-mir-205 would be downregulated in CFDP1_vs_CFDP2 based on macrophage transfer from monocytes [PMC4768678]. The results showed significant downregulation of both cfa-miR-30a and cfa-mir-205 in CFDP1_vs_CFDP2 condition [PMC4768678]. Lentiviral vectors were used to inhibit or overexpress cfa-mir-205 and inhibit NOTCH2 [PMC7860583]. The study predicted that several miRNAs, including cfa-mir-205, might regulate IgSF genes [PMC7689213].