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dre-mir-29a: Dre-mir-29a is a miRNA that has been shown to have age-dependent expression in previous studies [PMC5584509]. In experiments with zebrafish embryos, dre-mir-29a mimic was microinjected to investigate its regulation of Ireb2 mRNA [PMC5304403]. The 3'-UTRs of Ireb2 from zebrafish and N. furzeri were isolated and fused with the eGFP coding sequence to generate a reporter construct, which was then co-injected with dre-mir-29a mimic into zebrafish embryos [PMC5304403]. Dre-mir-29a was found to be one of the up-regulated miRNAs that potentially target down-regulated genes in a gene regulatory network [PMC4169864]. Additionally, dre-miR-22a-3p, dre-miR-16a, dre-miR-181a-3p, and dre-mir-29a were validated using qPCR in a study using RNA-seq data [PMC6684945]. Interestingly, the 10th cytosine of Pm-miR-29a was found to be replaced by uracil, similar to that in dre-mir-29a from Danio rerio and cte-miR-29a from Capitella teleta [PMC4691125]. Overall, these studies provide insights into the expression and regulatory roles of dre-mir-29a in various biological contexts.
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