cfa-mir-126: Cfa-mir-126 is a microRNA that has been found to possess higher diagnostic performance in discriminating the right or left divisional intrahepatic portosystemic shunt (IHPSS) group from the control group compared to other microRNAs such as cfa-miR-122, cfa-miR-34a, and cfa-miR-21 [PMC7356535]. It has been shown to have an area under the curve (AUC) of 1.00 in ROC analysis, indicating its strong diagnostic ability [PMC7356535]. Additionally, cfa-mir-126 has been found to be differentially expressed in both the RGC or RGC–CL groups compared to the control group, further supporting its potential as a diagnostic biomarker [PMC7356535]. ROC curve analysis has also demonstrated that cfa-mir-126 can be used as a diagnostic serum biomarker to differentiate splenocaval shunts from healthy controls [PMC7356535]. The optimal sensitivity and specificity for cfa-mir-126 have been determined at different cut-off values [PMC7356535]. Furthermore, cfa-mir-126 has been validated as one of the differentially expressed mature miRNAs in a negative miRNA–mRNA interaction network [PMC5844969]. Overall, these findings suggest that cfa-mir-126 is a promising non-invasive biomarker for various liver conditions and can reflect histopathological and molecular events occurring in different types of shunts with high sensitivity and specificity [PMC7356535][PMC5844969].