osa-miR160f-5p: Osa-mir160f-5p is a microRNA that is significantly down-regulated in DBA Aurora under all stresses, but up-regulated in L6 under heat stress (HS) and waterlogging stress (WH) [PMC7504575]. It has been found to target the chloroplastic PGR5-like protein, which is significantly up-regulated under HS and WH in both genotypes, with a higher fold-change in DBA Aurora under WH [PMC7504575]. The reduced abundance of osa-mir160f-5p and ata-miR396c-5p leads to increased expression of NDH and PGR5 in durum wheat [PMC9459240]. Osa-mir160f-5p is part of the osa-miR160 family, which targets seven members of the ARF gene family, including ARF4, ARF6, ARF8, ARF10, ARF16, ARF17 and ARF18 [PMC4257594]. In addition to osa-mir160f-5p, other members of the osa-miR160 family such as osa-miR167a-5p_R-1 and osa-miR167b_R-1 also target ARF6 and ARF8 [PMC4257594]. The expression levels of osa-miR167a-5p_R-1, osa-miR167b_R-1, osa-miR167c-5p_R-1, osa-miR160d-5p, osa-miR160e-5p and osa-mir160f were significantly higher in N2Y6 compared to LYP9 during grain-filling stages [PMC4257594]. This higher expression leads to decreased expression of ARF6 and ARF8, potentially repressing the production of jasmonic acid (JA) [PMC4257594]. Overall, osa-mir160f-5p plays a role in regulating the expression of target genes involved in stress response and hormone signaling pathways in durum wheat.