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ahy-miR160-5p: Ahy-mir160-5p is a microRNA that is down-regulated and accumulates in response to P deficiency stress. It targets four ARF genes, namely ARF16, ARF17, ARF18.1, and ARF18.2 [PMC9661748]. Under P deficiency stress, the transcription of ahy-mir160-5p and its target genes is altered, with the expression of ahy-mir160-5p being up-regulated and the target genes being down-regulated [PMC9661748]. This suggests that ahy-mir160-5p plays a role in regulating the peanut auxin signal transduction pathway in response to P deficiency stress [PMC9661748]. Additionally, it was found that the expression of ahy-mir160-5p was significantly up-regulated while its target genes were significantly down-regulated under -P treatment [PMC9661748]. The up-regulation of ahy-mir160-5p in response to P deficiency stress was observed in this study [PMC9661748]. Furthermore, it was discovered that the promoter region of ahy-mir160-5p contains an L3 element that can be bound by Dof11 transcription factors [PMC9661748]. In this study, other miRNAs were also found to be differentially expressed under -P treatment, with two miRNAs being up-regulated (ahy-miR3518 and ahy-mir160-5p) and four miRNAs being down-regulated (ahy-miR408-5p, ahy-miR408-3p, ahy-miR398, and ahy-miR3515) [PMC9661748].
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