cfa-mir-320: Cfa-mir-320, a microRNA, was found to be upregulated 1.43 times at 24 hours post-infection (hpi) [PMC9558963]. It was predicted to target the nuclear factor of activated T cells 1 (nfatc1) gene, which was associated with cytoplasm and cytoplasmic part in this study [PMC9558963]. The upregulation of cfa-mir-320 may be involved in resisting the invasion of T. canis larvae by inducing an inflammatory response or inhibiting T-cell function through targeting nfatc1 during early infection stages [PMC9558963]. Previous studies have shown that cfa-mir-320 is remarkably stable in serum, which is why it was included in the NGS-selected cmiRNA panel comprising other miRNAs [PMC9951723]. In the panel of candidate miRNAs for EC (endometrial cancer), cfa-mir-320 was elected as the best EC miRNA in dog serum [PMC9951723]. Furthermore, hsa-miR-320d, which has 100% homology with cfa-mir-320, has been identified as the most appropriate EC reference for normalizing circulating miRNA qPCR data [PMC9951723]. References: [PMC9558963] - Zhang et al. (2021). Identification and characterization of microRNAs involved in early infection stages of Toxocara canis larvae. Parasites & Vectors. [PMC9951723] - Zhang et al. (2022). Identification and characterization of circulating microRNAs as potential biomarkers for endometrial cancer in dogs. BMC Veterinary Research.