bta-mir-328: Bta-mir-328 is a microRNA that has been studied in relation to various aspects of cattle biology. It has been found to have a low ndG value compared to other microRNAs such as bta-miR-223 and lncRNA XR_003033296.1 [PMC8578396]. Bta-mir-328 is evolutionarily close to other microRNAs such as bta-miR-149-5p and bta-miR-185 in cow and wild yak, but distantly related to bta-miR-24-3p and bta-miR-874 [PMC8578396]. It has been shown to bind to three genes involved in a specific pathway [PMC8578396]. Bta-mir-328, along with other microRNAs like bta-miR-24-3p, bta-miR223, and bta-miR185, has been associated with bovine immune response [PMC8578396]. In a study comparing different stages of cattle development, the expression of bta-mir328 was found to be down-regulated in the BS stage compared with the CO stage [PMC9608168]. Bta-mir328 was also found to regulate target genes involved in cell division and growth [PMC9608168]. In another study comparing healthy cows with at-risk cows, several miRNAs including bta-mir328 were downregulated in healthy cows [PMC10000098]. One study identified an intersection between a predicted MRE for bta-mir328 and an annotated 3' UTR variant in SFTPA1 gene [PMC6061482]. The role of btmir328 in cattle is not well-studied but its human homolog has been shown to play a role in innate immune defense against Haemophilus influenza through negative regulation of phagocytosis [PMC6061482]. In a study comparing different time points, bta-mir328 was found to have a high betweenness in the downregulated miRNA group [PMC9445238].