cfa-let-7g: Cfa-let-7g is a highly expressed miRNA in various experimental groups [PMC9617701]. In a study involving testicular RA intervention, miRNA families such as miR-200, Mirlet-7, miR-125, miR-146, miR-34, miR-23, cfa-miR-184, cfa-miR-214, and cfa-miR-141 were significantly up-regulated [PMC4049822]. In another study investigating cardiac hypertrophy in dogs with heart diseases, cfa-miR-151 was upregulated in the PDA group and cfa-miR-30c was upregulated in the PDA and PS groups [PMC8542680]. Additionally, cfa-miR130b was upregulated in the MMVD group as well as the PDA and PS groups. Cfalet7g was also upregulated in the PDA and PS groups [PMC8542680]. When comparing stage C and D dogs with healthy controls, one microRNA (cfa-miR130b) was significantly up-regulated while eight microRNAs (cfa-miR375,cfa-miRNA425,cfa-mir30d,cfamir30c,cfamir151,cfale t7b,cfamir19b,andcfale t7g) were significantly down-regulated [PMC9607079]. These findings were determined using quantitative reverse transcription-polymerase chain reaction (qRTPCR) [PMC8542680] and a fold change cut-off of >1.5 with a p-value of <0.05 [PMC8062772].