bta-mir-106b: Bta-mir-106b is a microRNA that has been reported to be involved in various signaling pathways, including insulin, lipid, immune system, oxidative stress response, and muscle development. It is also associated with residual feed intake (RFI) in cattle [PMC9039951]. In a study using pmirGLO vectors carrying the 3′UTR sequence of CDKN1A, bta-mir-106b mimics and inhibitor were transferred into HEK293T cells [PMC9777812]. MicroRNAs are small non-coding RNA molecules that play important roles in gene regulation. Bta-mir-106b is one of the microRNAs that have been identified to have significant involvement in various signaling pathways. These pathways include insulin signaling, lipid metabolism, immune system regulation, oxidative stress response, and muscle development [PMC9039951]. Furthermore, bta-mir-106b has been found to be associated with residual feed intake (RFI) in cattle. RFI is a measure of feed efficiency and can impact the profitability of livestock production. The involvement of bta-mir-106b in RFI suggests its potential role in regulating energy metabolism and nutrient utilization [PMC9039951]. To further investigate the function of bta-mir-106b, a study utilized pmirGLO vectors carrying the 3′UTR sequence of CDKN1A (a gene involved in cell cycle regulation) and transferred them into HEK293T cells along with bta-mir-106b mimics and inhibitor. This experiment aimed to explore the regulatory effects of bta-mir-106b on CDKN1A expression [PMC9777812]. In conclusion, bta-mir-106b is a microRNA that plays important roles in various signaling pathways related to insulin signaling, lipid metabolism, immune system regulation oxidative stress response, and muscle development. It is also associated with residual feed intake in cattle. Further studies have investigated its regulatory effects on CDKN1A expression [PMC9039951] [PMC9777812].