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ccr-let-7a: Among the deposited miRNAs, 13 miRNAs (Ccr-miR-22a, ccr-miR-122, ccr-miR-146a, ccr-miR-192, ccr-miR-148, ccr-miR-100, ccr-miR-21, ccr-miR-126-3p, ccr-miR-143, ccr-miR-26a, ccr-let-7a, ccr-miR-101a, and ccr-miR-199-5p) were the most abundant miRNAs (TPM > 10,000) in both samples [1]. In primordial germ cells, miR-430-3p and miR-30-5p were the dominantly expressed conserved miRNAs, while ccr-miR-21 and ccr-let-7a were the dominantly expressed existing carp miRNAs in primordial gonads [2]. The detection of ccr-let-7a confirms that RNA quality is comparable in both samples [3]. Probes were designed to target ccr-miR-21, ccr-miR22a, ccr-let7a, and ccr-let7b as endogenous positive controls [3]. The integrity of miRNA was confirmed by analyzing ccr-let-7a in all samples [3]. The top 10 most abundant miRNA species include ccr-miR21 and ccr-let-7a [4].
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