bmo-mir-7: Bmo-mir-7 is a microRNA that has been studied in relation to its regulating relationships with Bmhairy [PMC4222307]. Western blotting analyses have shown that Bmo-mir-7 is a potentially fine-tuned target rather than a turn-on/turn-off target [PMC4222307]. The minimum free energy for hybridization has been determined to be -28.2 kcal/mol between BmEm4 and bmo-mir-7 and -17.6 kcal/mol between BmEm4 and bmo-mir-79 [PMC4222307]. A transfection vector containing the Bmyan 3'-UTR and the luciferase reporter gene was constructed, and luciferase activity analysis revealed a recognition site of bmo-mir-7 on the Bmyan 3'-UTR [PMC4222307]. The minimum free energy for hybridization between BmEm4 and bmo-mir-7 has also been predicted by mFold analysis to be -28.2 kcal/mol [PMC2760746]. The Drosophila orthologue of bmo-mir-7 has been identified in the nervous system, where it may play a role in segmentation, sensory organ development, and Notch signal transduction [PMC2835664]. The expression of bmo-mir-7 remains relatively stable throughout 14 development stages, with only weak increases observed at certain stages for bmo-miR-275 [PMC2500172]. Additionally, it has been found that certain homologs of six genes in Bombyx mori can bind perfectly to mir-2 (bmo-miR-2a, bmo-miR-2b, bmo-miR13a*, and bmo-miR13b) as well as to bmo mir-7 [PMC2435238].