cfa-let-7c: cfa-let-7c is a microRNA that has been identified as a potential regulator in canine biological processes, with TSC1, a gene encoding hamartin, predicted as one of its gene targets [PMC4490541]. This microRNA is among those with significantly different expression levels in dogs with different stages of cardiac disease, showing increased expression in stages B1/B2 or C/D compared to stage A [PMC4490541]. Specifically, cfa-let-7c was one of the top five meaningful miRNAs based on fold change and volume values and was suggested to be involved in the regulation of aging in dogs [PMC10135127]. Additionally, cfa-let-7c was among the miRNAs significantly up-regulated following testicular retinoic acid intervention [PMC4049822]. It was also validated to show significant differential expression between metastatic and non-metastatic mammary tumors [PMC7646326]. In the context of canine myxomatous mitral valve disease (MMVD), cfa-let-7c was upregulated in dogs with mild to moderate cardiac enlargement or congestive heart failure compared to normal dogs [PMC4964495], indicating its potential role as a biomarker for disease progression.