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aae-mir-100: Aae-mir-100 is an endogenous miRNA that has been used as an endogenous control in qPCR experiments to obtain expression profiles of anti-DENV miRNAs [PMC8668939]. It has been found to be highly expressed in CHIKV-infected Ae.albopictus saliva, along with aae-mir-125, aae-mir-263a, aae-mir-8, and aae-mir-184 [PMC4303268]. These same miRNAs were also highly expressed in Ae.aegypti saliva [PMC4303268]. In both Ae.albopictus and Ae.aegypti saliva, aae-mir-125 and aae-mir-100 were upregulated in CHIKV-infected samples [PMC4303268]. A target prediction analysis revealed that aae-mir-100 targets CDC42 and sumo-ligase [PMC7690852]. Additionally, the expression of aae-mir-100 was found to be significantly upregulated along with other miRNAs such as aae-miR-283 and aae-miR-305-3p [PMC7690852]. These miRNAs were also found to affect NK cell-mediated cytotoxicity and protein processing in ER pathways [PMC7690852].
In summary, the endogenous miRNA aae-mir-100 is used as an endogenous control in qPCR experiments to study anti-DENV miRNAs. It is highly expressed in CHIKV-infected Ae.albopictus saliva and is upregulated in both Ae.albopictus and Ae.aegypti saliva during CHIKV infection. A target prediction analysis revealed that it targets CDC42 and sumo-ligase. Additionally, the expression of aae-mir-100 was found to be significantly upregulated along with other miRNAs, and these miRNAs were found to affect NK cell-mediated cytotoxicity and protein processing in ER pathways. [PMC8668939] [PMC4303268] [PMC7690852]
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