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ath-miR396a-5p: Novel_6, novel_8, osa-miR167d-5p, mtr-miR319a-3p, ptc-miR396f, novel_9, ath-mir396a-5p, gma-miR396h, and zma-miR396g-3p were the most expressed DEMs in each sample [PMC9962977]. By using reverse-cDNA fragments as probes, we validated our bioinformatics prediction by northern blotting assay [PMC9203150]. The expression of ath-mir396a-5p was analyzed using qPCR with TaqMan miRNA assays [PMC8991712]. In the presence of ANE+NaCl, the expression of ath-mir396a-5p was higher after 12 hours compared to NaCl alone [PMC6205635]. Ath-mir396a-5p down-regulated the expression of AtGRF7, which is involved in salinity tolerance in Arabidopsis [PMC6205635]. In R lines, ath-mir396a-5p was downregulated at 2 dpi/6 dpi, leading to upregulation of its target gene EL10Ac5g11116 (chaperone protein) [PMC8786109]. Additionally, ath-mir396a-5p was found to co-regulate NFYA2 with ath-miR169a-5p and ath-miR169d [PMC7769420].
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