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bmo-mir-14: Bmo-mir-14 is a miRNA that has been studied in the context of the interaction between silkworm and BmCPV. It has been found that the expression level of bmo-mir-14 is down-regulated in the infected midgut at 72 h post inoculation, suggesting its potential role in this interaction [PMC3699532]. In a study using qRT-PCR, bmo-mir-14 was validated as one of the down-regulated miRNAs [PMC3699532]. However, contrary results were found for bmo-mir-14 in terms of its expression levels when compared to literature-based collections [PMC4045974]. Bmo-mir-14 has been shown to have a synchronized expression pattern with bmo-miR-10 and they share common targets such as ecdysone receptor (EcR), orphan nuclear receptor (E75), BmCF1, and Jhe [PMC2500172]. Additionally, bmo-miR-278 has been found to have a relatively high expression level and may play a similar role as bmo-miR-10 and bmo-mir-14 in regulating energy metabolism at molting stage [PMC2500172]. Bmo-mir-14 was also strongly expressed in all developmental stages (larva, pupa, and moth) [PMC2435238]. In terms of experimental techniques, methylated DNA probes were used to improve signal strength for detecting bmo-mir-14 on Northern blots [PMC2435238]. Furthermore, over-expression of bmo-miR-9a, bmo-mir-14, and bmo-miR2766 resulted in a significant decrease in the relative expression of the reporter gene Renilla luciferase [PMC3535529].
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