bta-mir-335: Bta-mir-335 is a miRNA that regulates PRLR and is involved in linseed oil-treated cows [PMC9821774]. In linseed oil-treated cows, bta-mir-335, miR-200a, and bta-miR-2299-5p are down-regulated and target 1163 genes [PMC4628385]. In addition, bta-mir-335 is one of the most significant DE-bta-miRNAs that target 3'UTR of 273 genes with high confidence [PMC9437019]. Bta-mir-335 is not detected in granulosa cells of DF [PMC4156418]. Bta-mir-335 is one of the differentially expressed miRNAs in theca cells during the estrous cycle [PMC4156418]. The expression pattern of selected miRNAs in theca cells shows similarity to that of granulosa cells in SF compared to DF, except for miR-2487 and bta-mir-335 [PMC4156418]. The expression trend of selected miRNAs in theca cells at day 3 of the estrous cycle is similar to that of corresponding granulosa cells except for bta-miR-34c and bta-mir-335 [PMC4156418]. Bta-mir-335 is one of the nine differentially expressed candidate miRNAs validated by qPCR [PMC4438052]. Bta-mir-335 shows higher relative abundance in subordinate follicles compared to preovulatory dominant follicles in terms of follicular fluid and theca cells [PMC4438052]. Furthermore, preovulatory dominant follicles exhibit reduced expression levels for other matured miRNAs including bta-miR409a, bta-miR378, and btm-MIR17.5p [PMC4438052].