dme-mir-277: Dme-mir-277 is a microRNA found in Drosophila melanogaster [PMC5459504]. Inhibition of dme-mir-277 using miRNA sponge constructs has been shown to increase endogenous muscle blind levels [PMC6888406]. Inhibition of dme-mir-277 resulted in a 15% reduction in IFM area compared to control flies [PMC5090246]. Silencing dme-mir-277 or dme-miR-304, another 3′UTR translation repressor, increased muscleblind levels and improved survival in DM1 flies [PMC5090246]. The silencing of dme-mir-277 or dme-miR-304 also resulted in changes to muscleblind subcellular localization and increased cytoplasmic Mbl levels [PMC5090246]. The binding sites of dme-mir-277 and dme-miR-304 overlap in the mblD 3′ UTR, which may explain the difference observed between in vivo and luciferase assays for mblD regulation by dme-mir-277 [PMC5090246]. Silencing of both microRNAs also rescued missplicing events associated with DM1 flies [PMC5090246]. The upregulation of muscleblind achieved by silencing these microRNAs has also been shown to reduce muscle atrophy and improve motor function and lifespan in DM1 flies [PMC5090246] [PMC6321436] [PMC8634727].