ssc-mir-326: ssc-mir-326 is a microRNA (miRNA) identified in pigs that is differentially expressed in liver tissue under normoxic and hypoxic conditions and is associated with pathways relevant to cancer and focal adhesion [PMC8861501]. This miRNA, particularly the rs319154814 (G/A) polymorphism within its apical loop, has been linked to the expression levels of its target mRNAs, with significant associations found after multiple testing corrections [PMC8097994]. Notably, pigs homozygous for the A-allele of this polymorphism exhibited an approximate 1.9-fold increase in ssc-mir-326 expression, although this increase was not statistically significant [PMC8097994]. The ssc-mir-326 genotype also correlates with the expression of circadian clock regulatory genes such as HDAC3 and PER1 [PMC8097994]. Furthermore, this miRNA polymorphism has been associated with lipid traits in pigs [PMC8097994]. The hepatic mRNA targets most significantly associated with the ssc-mir-326 genotype include CFLAR, PPP1CC, SDC1, SF3A3, and FSTL1 [PMC8097994], suggesting a potential role for ssc-mir-326 in lipid metabolism pathways despite no direct effects on myristic fatty acid metabolism being described to date [PMC8097994]. The rs319154814 (G/A) variant may enhance the repressive activity of ssc-mir-326 on its mRNA targets such as PPP1CC [PMC8097994], indicating a functional impact of this polymorphism on gene regulation.