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MIR9-3: MIR9-3 is one of the 12 common genes that showed methylation in both primary tumors associated with metastasis and advanced stages [PMC8835734]. The methylation status of the three MIR9 promoters, including MIR9-3, was analyzed using Methylation Specific PCR (MSP) [PMC5366901]. The primer sets used in the analysis were designed based on the CpG region encompassing the transcription start sites of MIR9 genes, which were validated by RACE [PMC5366901]. MIR9-1, MIR9-2, and MIR9-3 are located on chromosomes 1, 5, and 15 respectively [PMC9220270].
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