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gga-mir-20b: Gga-mir-20b is a microRNA that is regulated by FOXO3, a major regulator of oxidative stress and metabolic pathways, and its expression is significantly higher in delay-fed birds [PMC7936154]. The expression of gga-mir-20b in the liver is downregulated after hatching and is significantly lower in fed-from-hatch chicks compared to delayed-fed chicks [PMC7936154]. Gga-mir-20b has been found to target lipid metabolism-associated genes such as MSMO1 and FADS1 [PMC7936154]. The upstream region of gga-mir-20b was obtained from Ensembl, and promoter-like elements were predicted using PROSCAN [PMC5583987]. A bait strain containing the gga-mir-20b promoter cassette was generated following the manufacturer's instructions [PMC5583987].
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