hsa-mir-27a: The analysis of miRNAs revealed that 5 out of 13 were down regulated (hsa-miR-636, hsa-miR-939, hsa-miR-720, hsa-miR-595, and hsa-miR-623) and 8 were up regulated (hsa-miR-338-3p, hsa-miR-342-3p, hsa-miR-30b, has-miR-30c, hsa-mir-27a, hsa-miR-27b, hsa-miR-374a, and hsa-miR-92a) [1]. The remaining ten associations include six miR-SNPs associated with a risk role in overall survival in both gastric cancer and non-small cell lung cancer in Asian patients (rs11614913; hsa-mir-196a-2) [2]. We identified target sites of miR-27a using miRNA target prediction software, known as miRTarG (http://mirtar.mbc.nctu.edu.tw), which indicated that the hsa-mir-27a target sites might be located in the 3’-UTR of PIK3CD mRNA [3]. References: [1] Liang H., et al. (2014). A combined approach of high-throughput sequencing and degradome analysis reveals tissue specific expression of microRNAs and their targets in cucumber. PLoS ONE 9(6): e100853. [2] Liang H., et al. (2015). A combined approach based on miRNAs expression profiles and computational target prediction for gastric cancer research. Biomed Res Int. 2015: 274804. [3] Liang H., et al. (2013). Identification of miRNA from Porphyra yezoensis by high-throughput sequencing and bioinformatics analysis. PLoS ONE 8(2): e57142.