eca-mir-30d: Eca-mir-30d, a microRNA, was identified as the only miRNA in the dataset that qualified as an endogenous control [PMC8699634]. The relative quantification of candidate miRNAs was performed using the 2-ΔΔCq method, with eca-mir-30d serving as the endogenous control [PMC8699634]. In an exploratory NGS study, eca-mir-30d was found to have the lowest coefficient of variation (CV = 15.6) and was selected as the endogenous control [PMC8699634]. In an equine model of severe asthma, several miRNAs including eca-mir-30d were found to be differentially expressed in serum and were implicated in regulating airway remodeling and CD4+ T cell maturation and differentiation [PMC8372030]. Using DESeq2 analysis, 11 miRNAs including eca-mir-30d were identified as statistically significant differentially expressed miRNAs after accounting for hemolysis levels [PMC5748701]. Eca-mir-30d was selected as an endogenous control alongside cel-miR-39-3p as an exogenous control based on previous recommendations [PMC9873782].