cfa-mir-197: Cfa-mir-197 is a microRNA that specifically binds to the 3′-UTR of KPNA6, mediating its silencing [PMC8779696]. In MDCK cells infected with influenza B virus, including the Victoria and Yamagata lineages, cfa-mir-197 is significantly upregulated [PMC8779696]. Luciferase reporter assays confirmed that KPNA6 is a putative target of cfa-mir-197 in MDCK cells [PMC6893747]. The expression of cfa-mir-197 and cfa-miR-215 is increased during influenza B virus infection [PMC6893747]. Overexpression of cfa-mir-197 reduces KPNA6 expression at the mRNA level, while inhibition of cfa-mir-197 leads to increased expression of KPNA6 and PB1 [PMC6893747]. The luciferase activity assays demonstrate that KPNA6 contains putative binding sites for cfa-mir-197 but not for cfa-miR-215 [PMC6893747]. Computational programs were used to predict target genes regulated by cfa-mir-197 and cfa-miR215 [PMC6893747]. The expression levels of both microRNAs are significantly higher in cells infected with the Yamagata lineage compared to the Victoria lineage [PMC6893747]. These findings suggest that KPNA6 may be directly regulated by both microRNAs, influencing influenza B virus replication [PMC6893747]. Overexpression of cfa-mir-197 leads to downregulation of KPNA6 and viral PB1 expression at later time points during infection [PMC6893747].