bta-mir-376a: Bta-mir-376a is a microRNA that plays a role in the regulation of preadipocyte proliferation and differentiation in Qinchuan beef cattle [PMC7763857]. The CCK-8 findings showed that the overexpression of bta-mir-376a inhibited the proliferation of adipocytes [PMC7763857]. The expression levels of bta-mir-376a were higher in the stomach and reticular tissues compared to other tissues [PMC7763857]. Bta-mir-376a was found to target the 3'-UTR of KLF15, a gene involved in adipogenesis, as predicted by bioinformatics tools [PMC7763857]. Dual-luciferase reporter assays confirmed KLF15 as a target gene of bta-mir-376a [PMC7763857]. Bta-mir-376a was found to be a negative regulator of adipocyte differentiation and adipogenesis signal transduction pathways [PMC7763857]. The overexpression of bta-mir-376a reduced triglyceride contents and downregulated the expression of adipogenic markers such as CEBPα, PPARγ, and FAS genes [PMC7763857]. Bta-mir-376a inhibited adipocyte proliferation by reducing S-phase cells and decreasing EdU-labeled cells [PMC7763857]. Staining with oil red O confirmed that bta-mir-376a overexpression reduced lipid droplets in bovine adipocytes [PMC7763857]. The expression levels of bta-mir-376a increased during the proliferation stage but decreased during differentiation, suggesting its role in regulating both processes simultaneously [PMC7763857]. Overall, bta-mir-376a is a negative regulator that inhibits preadipocyte proliferation and differentiation by targeting KLF15. It plays an important role in regulating bovine adipogenesis.