bta-mir-221: Bta-mir-221 is a microRNA that has been studied in various contexts. It has been found to be significantly regulated between pregnancy and the oestrous cycle on day 4, as well as between cycle and pregnancy from day 4 to 18 [PMC5325256]. In a study using MC sequencing data, the Day 18/Day 4 ratios of bta-mir-221 were used to discriminate between pregnant and cyclic animals [PMC5325256]. Bta-mir-221, along with other miRNAs, was validated using RT-qPCR [PMC5325256]. It has also been found to be highly enriched in the follicular fluid of subordinate follicles compared to dominant follicles [PMC4438052]. Bta-mir-221 is one of the miRNAs that were differentially expressed in theca cells during the estrous cycle [PMC4156418]. In bovine colostrum and mature milk exosomes, bta-mir-221 showed significantly higher expression compared to other miRNAs [PMC8203993]. It has also been associated with regions with dam-truncated transcript regions (TRD) and is involved in VEGF-induced endothelial cell activation pathway during angiogenesis [PMC9778079]. Bta-mir-221 is one of the many known miRNAs that have been identified in various studies [PMC5821052]. Additionally, it was found to be differentially expressed in comparison groups such as H_SCM, ARM_SCM, and H_ARM [PMC10000098]. References: [PMC5325256] [PMC4438052] [PMC4156418] [PMC8203993] [PMC9778079] [PMC5821052] [PM10000098]