MIR499B: MIR499B is a non-coding RNA gene that is transcribed from the reverse strand of the same genomic region as MIR499A [PMC5591547]. Multiple sequence alignment and phylogenetic tree construction were used to identify similarity regions in different species, including MIR499B [PMC5591547]. Variant analysis of MIR499A revealed the presence of 30 non-coding transcript exon variants, including 19 overlapping variants in MIR499B [PMC5591547]. Computational tools predicted that miR-499a and MIR499B target key molecules in asthma-related KEGG pathways [PMC5591547]. Bioinformatics analysis showed a common polymorphism within the seed region of mature miR-499a-3p in both MIR499A and MIR499B genes [PMC5591547]. The MIR499A gene is located within intron 19 of the MYH7B gene, while MIR499B forms a shorter transcript from the reverse strand [PMC5591547]. Hsa-miR-499b is another miRNA precursor encoded from the opposite strand of the same region as MIR499A and processed into hsa-miR-499b-5p and hsa-miR-499b-3p [PMC6359604]. The rs3746444:T>C polymorphism in both mature MIR499A and seed of MIR499B was found to be inversely associated with cardia localization of adenocarcinoma [PMC6687864]. This polymorphism may influence processing and expression of both genes, potentially affecting their target genes in a dosage-dependent manner [PMC6687864]. Associations were found between different gastric adenocarcinoma subtypes and several single nucleotide variants (SNVs), including rs3746444:T>C in the seed of MIR499A and mature MIR499B [PMC6687864].