bta-mir-494: Bta-mir-494 is a regulator of PTEN in follicular cells and is involved in the PI3K-Akt signaling pathway [PMC5602670]. It is a microRNA that plays a role in various cellular processes such as protein synthesis, DNA repair, cell cycle progression, and apoptosis [PMC5602670]. In follicular cells with high oocyte developmental potential, there are lower levels of PTEN, FOXO3a, bta-mir-494, bta-miR-20a, and BAX [PMC5602670]. Bta-mir-494 regulates the levels of PTEN in follicular cells [PMC5602670]. The levels of bta-mir-494 and bta-miR-20a are significantly higher in follicular cells derived from follicles with cleaved oocytes compared to those without cleaved oocytes [PMC5602670]. The interaction between bta-mir-494 and the bovine PTEN 3’UTR has been experimentally validated using a luciferase assay [PMC5602670]. In poor oocyte quality groups, there are higher levels of PTEN mRNA and lower levels of bta-mir-494 and bta-miR-20a [PMC5602670]. Bta-mir-494 has also been detected in extracellular vesicles from various fluids such as ovarian fluid and uterine fluid [PMC9594899]. It has been associated with implantation in mice [PMC9594899]. In response to Gram-positive S. uberis infection, the expression of bta-mir-494 is down-regulated while it is up-regulated in response to LPS stimulation. This inverse response has also been observed for other microRNAs such as bta-miR-100 [PMC3589390].