bta-mir-615: Bta-mir-615 is one of the 13 highly abundant miRNAs identified in the study, with Ct numbers between 10-25 Ct [PMC5472319]. The study aimed to evaluate the molecular effects of different culture systems on post-transcriptional regulators, and bta-mir-615 was investigated as a biomarker for embryo quality and development [PMC10121032]. The expression of bta-mir-615 was found to be decreased in the LM group compared to the control group [PMC10121032]. The RT-qPCR method was used to measure bta-mir-615 expression, with specific primers and reagents [PMC10121032]. Additionally, bta-mir-615 was found to be one of the top four miRNAs enriched in extracellular vesicles from both fluids [PMC9594899]. Furthermore, bta-mir-615 is a tissue-specific miRNA that is expressed in rumen tissue [PMC6371894]. Overall, these findings suggest that bta-mir-615 may play a role in embryo development and quality assessment, as well as being involved in intercellular communication through extracellular vesicles. Further research is needed to fully understand its specific functions and mechanisms.