ame-let-7: Ame-let-7 is a miRNA that is differentially expressed and plays a critical role in cell signaling pathways in various species [PMC4704047]. In honeybees, queen larvae express ame-let-7 twice as much as worker bee larvae, while the expression of ame-miR-263 in queen larvae is only one fifth of that in worker bee larvae [PMC6942390]. Ame-let-7 interacts with genes involved in the NF-κB immune signaling pathways, regulating the production of antimicrobial peptides for cellular defense response [PMC8532292]. Ame-miR-100, ame-miR-125, and ame-let-7 are highly expressed in honeybee wing discs and are located together on the LG8 linkage group of the honeybee genome [PMC8532292]. Other examples of clustered or multicopy miRNAs include ame-mir-137 overlapping with novel miRNA C5303 and ame-mir9b overlapping with ame-mir79 on the opposite strand [PMC2394756]. Ame let 7 is found on the same scaffold as ame mir 100 [PMC2394756]. Four miRNAs commonly found in other animals or products, including milk, are also present in royal jelly (RJ) - these include ame let 7, mir 34, mir 100 and mir 375 [PMC3424160]. In this study, it was found that males have higher expression levels of both ame-miR9a and ame let 7 compared to females suggesting their important roles in male development [PMC6835379].