aly-miR166a-3p: Aly-mir166a-3p is a plant miRNA that shows strong fluid/tissue specificity, as observed in Fig 5 [PMC6021041]. It is involved in the regulation of HD-ZIP transcription factors, such as BraA06g002210.3C, BraA08g002260.3C, and BraA09g034560.3C [PMC10039531]. These transcription factors are targeted and regulated by aly-mir166a-3p, along with other miRNAs such as ath-miR166a-3p, ath-miR166a-3p_1ss21CA, bna-miR166a, bna-miR166f, cas-miR166e, gma-miR166a-3p_R + 1, and mtr-miR166a_R + 1 [PMC10039531]. In experimental evaluation of genes related to apoptosis in broccoletti plants (Brassica oleracea var. italica), the miRNAs bra-miR156g-5p and Myseq-330 (similar to aly-mir166a-3p) were found to be the most corresponding broccoletti miRNAs [PMC7147085]. Aly-mir166a-3p is a member of the MIR166 family and has been identified as one of the most highly upregulated miRNAs [PMC9010791]. In qPCR analysis of known miRNAs in alfalfa roots (Medicago sativa), aly-mir166a-3p was found to be down-regulated along with other miRNAs such as aqc-miR166a and hbr-miR166a [PMC5406866]. These findings highlight the importance of aly-mir166a-3p in plant development and gene regulation.