bmo-mir-9a: Bmo-mir-9a is a conservative miRNA [PMC4222307]. Over-expression of bmo-mir-9a, bmo-miR-14, and bmo-miR-2766 resulted in a significant decrease in the relative expression of the reporter gene, Renilla luciferase [PMC3535529]. The precursors of bmo-miR-14, bmo-miR-2766, and bmo-mir-9a were amplified from the B. mori genome DNA and inserted into pmR-mCherry for miRNA expression [PMC3535529]. Using qRT-PCR, it was confirmed that bmo-mir-9a was down-regulated in sequence data along with other miRNAs [PMC3699532]. Bmo-miR-8, bmo-mir-9a, and bmo-miR-263a showed a slight elevation after the diapause-broken stage and maintained a relatively constant level thereafter [PMC2500172]. The down-regulation of Bmase gene expression was attributed to the regulation function of bmo-mir-9a [PMC4222307]. A target-binding site for bmo-mir-9a was identified in the 3'UTR of Bmase gene [PMC4222307]. Co-transfection experiments with synthetic mimics of bmo-mir-9a and Bmase 3'UTR fused luciferase reporter plasmid confirmed these results [PMC4222307]. A vector over-expressing bmo-mir 9-a and a reporter plasmid with Bmase 3'UTR fused to firefly luciferase gene were constructed to further verify the regulation function of bno mir 9-a on Bmase gene expression. These findings highlight the role of mir 9-a in down-regulating Bmase gene expression [PMC4222307].