bta-mir-346: Bta-mir-346 is a microRNA that is not detected at day 3 but increased at day 7 of the estrous cycle [PMC4156418]. It is one of the miRNAs that showed differential expression during the estrous cycle, with two upregulated miRNAs (bta-miR-374b, bta-miR-2887) and two downregulated miRNAs (bta-miR-147, bta-mir-346) consistently observed [PMC8568169]. Interestingly, bta-mir-346 was significantly found in subclinical stages before clinical signs appeared [PMC8568169]. Additionally, a human homolog of bta-mir-346 has been identified as a potential biomarker for tuberculosis and M. avium complex pulmonary disease caused by Mycobacterium species [PMC8568169]. Bta-mir-346 has the longest region of consecutive polymorphisms among microRNAs, with six out of seven nucleotides in its seed region being polymorphic [PMC4379384]. Overall, these findings suggest that bta-mir-346 plays a role in the regulation of gene expression during the estrous cycle and may have potential implications in disease biomarker research.