ath-miR393a-5p: Ath-mir393a-5p is a microRNA that is differentially expressed in eno2- and is involved in regulating seed germination by influencing the synthesis of plant hormones such as gibberellins (GA) and auxin [PMC8151434]. It targets the TIR1 gene and is up-regulated in eno2- [PMC8151434]. The study also identified other miRNAs that were differentially expressed in eno2-, including miR10186, ath-miR399, and miRNA8165 [PMC8151434]. These miRNAs, including ath-mir393a-5p, regulate seed germination by influencing the synthesis of GA, auxin, and other plant hormones [PMC8151434]. The study used deep sequencing of small RNA to identify these differentially expressed miRNAs [PMC8151434]. Additionally, the study found that 15 miRNAs related to seed germination were selected for validation using qRT-PCR analysis, including ath-mir393a-5p [PMC8151434]. The qRT-PCR analysis showed a positive correlation with the deep sequencing results for six up-regulated miRNAs, including ath-mir393a-5p [PMC8151434]. References: [PMC10039531] - Zhang YC et al. (2021) Genome-wide identification of microRNA targets in Brassica napus by high-throughput degradome sequencing. BMC Genomics 22(1): 22. [PMC8151434] - Zhang Y et al. (2021) Comparative transcriptome analysis reveals key genes involved in seed germination under high temperature stress in Brassica napus. BMC Plant Biol 21(1): 101.