ame-mir-263b: Ame-mir-263b, a significant miRNA found in Macrobrachium nipponense, was absent in a previous study by RJM [PMC3424160]. In a study by Ashby et al., Ame-mir-263b was used as a reference sequence [PMC5452642]. Ame-mir-263b was observed to be induced in G. mellonella during pupation, similar to its expression profiles in B. mori [PMC4156658]. It specifically showed induction in pupae but not pre-pupae and targeted mRNAs encoding dead box polypeptide 1 and cd27-binding protein isoform 1 [PMC4156658]. However, its predicted target contig 20004 was upregulated rather than downregulated during metamorphosis [PMC4156658]. Ame-mir-263b, along with aca-miR-30b-5p and cfa-miR-125a, may have a strong influence on sex-differentiation and sex-determination in M. nipponense [PMC5607309]. Among the three miRNAs studied, ame-mir-263b had the highest expression level in the androgenic gland [PMC5607309]. In the ovary, ame-mir-263b was up-regulated by 3.37-fold compared to control samples [PMC5607309]. A total of 11 differentially expressed genes (DEGs) predicted as target genes of ame-mir-263b were identified [PMC5607309].