RNY5: RNY5 is a highly conserved human YRNA that is known to contain a 5' end 8 nt motif (GUAGUGGG) and a 3' end 9 nt motif (CCCACUGCU) [PMC8973356]. It is the most abundant miscRNA gene present in extracellular vesicles (EVs), composing a significant proportion of sRNAs in both BJ EVs (35%) and K562 EVs (48%) [PMC4604435]. Interestingly, the decrease in RNY5 expression in EVs was not accompanied by an increase in the number of full-length RNY5 reads [PMC7430647]. This suggests that there may be alternative processing or degradation mechanisms affecting RNY5 within EVs. Furthermore, experiments have shown that mutations in RNY5 can lead to changes in its internal loops and stem domains [PMC8973356]. Overall, these findings highlight the importance of RNY5 as a highly abundant YRNA present in EVs and suggest that it may have functional roles beyond its known involvement in DNA replication initiation.