BMF-AS1: BMF-AS1 is a long non-coding RNA (lncRNA) that is transcribed from the negative strand of the BMF gene [PMC9937703]. It has been found to play a role in the calcification and senescence of vascular smooth muscle cells (VSMCs) [PMC9937703]. BMF-AS1 regulates the actin-binding protein BMF and promotes high glucose-induced VSMC calcification and senescence [PMC9937703]. It interacts with BMF at both the mRNA and protein levels, upregulating its expression [PMC9937703]. Knocking down BMF-AS1 accelerates the degradation of BMF mRNA in VSMCs, while overexpression of BMF-AS1 protects it [PMC9937703]. In VSMCs induced by high glucose and aorta tissues from diabetic mice, both BMF-AS1 and BMF expression are increased [PMC9937703]. Overexpression or knockdown of either BMF or BMF-AS1 affects VSMC calcification and senescence, with overexpression exacerbating it and knockdown attenuating it [PMC9937703]. The expression of BMF-AS1 is regulated by histone deacetylase inhibitor TSA but not by 5-aza-2’-deoxycytidine, a methyltransferase inhibitor [PMC9937703]. The location of BMF-AS1 is mainly in the cytoplasm but also at a low level in the nucleus of VSMCs, with high glucose treatment increasing its expression [PMC9937703]. The detailed mechanisms underlying its regulation in VSMC calcification and senescence require further exploration [PMC9937703]. Reference: [PMC9937703]