aga-mir-14: aga-mir-14 is a microRNA that was selected for functional study based on its predicted targeting of multiple immune genes and its identification as the most abundant miRNA candidate using the CLEAR-CLIP approach [PMC7202664]. Depletion of aga-mir-14 was found to result in the suppression of both parasite species and the microbiota, indicating its role in regulating diverse immune genes and modulating broad-spectrum defenses [PMC7202664]. Specifically, depletion of aga-mir-14 led to a significant increase in the mRNA abundance of Tep1, a key anti-Plasmodium factor, in mosquito midguts [PMC7202664]. While hundreds of genes were regulated upon depletion of aga-mir-14 or aga-miR-305, only a few contained potential binding sites for these miRNAs [PMC7202664]. The sponge for aga-mir-14 was predicted using miRNAsong by introducing mismatches at specific positions [PMC7202664]. Conditional depletion of aga-mir-14 resulted in suppression of both P. falciparum and P. berghei infections, as well as midgut microbiota [PMC7202664]. Additionally, a novel miRNA called aae-mir-143 with a seed sequence match to aga-mir-14 was identified as highly expressed [PMC4303268]. Depletion of both aga-mir-14 and aga-miR305 increased mosquito resistance to P. berghei and P. falciparum infections by enhancing the expression of multiple immunity-related and anti-plasmodium genes [PMC8716737]. Overall, these findings highlight the role of miRNAs such as aga-miR8, aga-miR305, and particularly aga-mir14 in regulating mosquito immunity against parasite infections [PMC8716737].