zma-miR169o-5p: Zma-mir169o-5p is a miRNA that negatively regulates eight genes, including GRMZM2G165488, GRMZM2G134396, GRMZM2G133568, GRMZM2G098594, GRMZM2G105335, GRMZM2G033230, GRMZM2G399072, and GRMZM2G009871 [PMC9604548]. In addition to zma-mir169o-5p, there are five other miRNAs that target these genes: zma-miR390a-5p, zma-miR396c, zma-miR397b-p5, zma-miR444a and novel-miR4 [PMC9604548]. The expression of these miRNAs is negatively related to the expression of their target genes [PMC9604548]. Zma-mir169o-5p is specifically related to seed storability germination and is down-regulated with the decrease of seed vigor [PMC9604548]. The target gene for zma-mir169o-5p is GRMZM2G165488 [PMC9604548]. GO enrichment analysis revealed that there are 13 pairs of negatively regulated target genes corresponding to miRNAs highly correlated with seed storability. Nine miRNAs were down-regulated (including zma-mir169o-5p) and four were up-regulated (including zma-miR444a) [PMC9604548]. After artificial aging experiments on two inbred lines with differential expression of certain miRNAs including zma-mir169o-5p and zma-miR444a were found to be negatively correlated with seed storability. On the other hand, the expression of miR444a was found to be positively correlated with seed storability [PMC9604548]. The presence of zma-mir169o-5p, zma-miR390a-5p, zma-miR396c, zma-miR444a, zma-miR397b-p5, and novel-miR4 was identified through high-throughput sequencing and these miRNAs may play a regulatory role in seed storability [PMC9604548].