rno-mir-489: Rno-mir-489 is a down-regulated microRNA (miRNA) that is associated with the up-regulation of mRNA expression of its predicted target gene, Bcl10 [PMC2974699]. It is also predicted to regulate a vast number of genes, making speculation on its function difficult [PMC3386237]. Rno-mir-489 is present within the Calcr gene [PMC3386237]. Its expression is most noticeable in the theca and cumulus cells of FC [PMC3682887]. In a study examining aberrantly expressed miRNAs, rno-mir-489 was found to be down-regulated in samples from animals treated with 2-mg/kg [PMC2974699]. Another down-regulated miRNA, rno-let-7e*, was also consistent with the up-regulation of its predicted target gene, Ccna2 [PMC2974699]. Rno-mir-489 and rno-let-7e* were found to be predicted targets of Ccna2 and Bcl10, respectively [PMC2974699]. In addition to rno-mir-489, rno-miR653 was also found within the Calcr gene [PMC3386237]. These findings suggest that rno-mir-489 may play a role in regulating gene expression and could be involved in various biological processes. However, further research is needed to fully understand its function.