rno-mir-499: Rno-mir-499 is a microRNA that is down-regulated in the heart of injured animals, as observed in the data [PMC6377737]. Interestingly, it is up-regulated in the lung of injured animals, along with rno-miR-208a and rno-mir-499-5p [PMC6377737]. In a study comparing heart failure (HF) and control groups, rno-miR-1-3p, rno-let-7 family, rno-miR-29a-3p, rno-miR-133a-3p, rno-mir-499-5p and rno-miR-140 were found to be highly expressed in both groups [PMC4978447]. This finding is consistent with previous studies that have shown high expression of rno-miR133a, rno-miR1 and rno-mir499 in the heart [PMC4978447]. In another study on kidney stone formation, it was found that the expression of rno-miR192, rnomiR194 and rn0mir499 was higher in the stone-forming group compared to their potential target gene chemokine receptor 2 (CCR2) [PMC5748115]. This suggests that overexpression of CCR2 mediated by these miRNAs could induce inflammation and damage to renal tubular epithelial cells and promote nephrolithiasis [PMC5748115]. The synthesis of complementary DNA was performed using High-Capacity cDNA reverse transcription kits according to the manufacturer's instructions with respective primers for rn0mir499 [PMC3515561]. The primers used for rn0mir499 were: sense: 5′GGGGTTAAGACTTGCAGTG′3′ and antisense: 5′CAGTGCGTGTCGTGGAGT′3′ [PMC3515561].