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mmu-mir-327: Mmu-mir-327 is a microRNA that is least homologous to bta-mir-2422, rat rno-mir-327, and mouse mmu-mir-327 [PMC6722470]. The primers used for amplification of miR-327 were mmu-mir-327: 5′-ACUUGAGGGGCAUGAGGAU-3′ and rno-miR-327: 5′-CCUUGAGGGGCAUGAGGGU-3′ [PMC5810199]. Custom miRCURY in vivo LNA inhibitor probes were used against mmu-mir-327, with a LNA inhibitor control serving as a negative control [PMC5727036]. Transfection experiments were conducted using ON-TARGETplus SMARTpool siRNAs against Fgfr2 and Fgf10, miRIDIAN mmu-mir-327 mimic, mmu-mir-327 miRCURY LNA inhibitor, and their respective negative controls at a final concentration of 25 nM [PMC5727036]. An adenovirus overexpressing mmu-mir-327 was injected into scWAT and visWAT [PMC5727036]. Among the significantly differentially expressed miRNAs, mmu-miR1223p, mmu-miR6238, mmu-miR669a3p, mmu-miR691, mmu-miR2233p, mmumir327 ,mmummiR207 ,andmmummiR466f3p were upregulated whilemmummiR30f ,mmummiR344b5p ,mmummiR28b ,mmummi R2963p ,mmumm i R70623 p,and m mumm i R30905 p were downregulated [PMC5819906].
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