mmu-mir-599: Mmu-mir-599 is a type of microRNA that was transfected into Hepa1-6 cells for gain and loss-of-function experiments [PMC8846938]. Transfection with mmu-mir-599 mimic reduced PXR 3'UTR-dependent luciferase activity [PMC8846938]. Mice treated with resveratrol showed reduced mmu-mir-599 expression and increased PXR expression [PMC8846938]. The online database miRDB was used to verify the sequence of mmu-mir-599 and predict PXR as a potential target gene [PMC8846938]. The full-length coding sequences of PXR were amplified and subcloned into a plasmid for further analysis [PMC8846938]. The study aimed to investigate the therapeutic role of resveratrol in NASH and its effects on mmu-mir-599, PXR, and related inflammatory genes [PMC8846938]. The binding site of mmu-mir-599 in the PXR mRNA sequence was confirmed through luciferase activity assay with site mutation [PMC8846938]. Mmu-mir-599 mimic suppressed the mRNA and protein expression levels of PXR, while the inhibitor had the opposite effect in Hepa1-6 cells [PMC8846938]. Resveratrol downregulated mmu-mir-599 expression but upregulated mRNA and protein expression levels of PXR in NASH model mice and Hepa1-6 cells [PMC8846938]. Mmu-mir-599 expression was increased in NASH model mice, suggesting its potential role in NASH development through inhibiting PXR expression at the transcriptional level [PMC8846938]. Resveratrol's anti-inflammatory effects may be mediated by mmu-mir-599, but further investigation is needed to understand the detailed mechanisms involved [PMC8846938].