hsa-mir-3658: Hsa-mir-3658 is a microRNA that has been implicated in interacting with several other microRNAs, including hsa-miR-16-1-3p, hsa-miR-4698, hsa-miR-27a-3p, hsa-miR-27b-3p, hsa-miR-3662, hsa-miR-5571-5p, hsa-miR-570-3p, hsa-mir3658 itself, and others [PMC5828204]. The relative expression levels of several microRNAs, including hsa-mir3658, were calculated using the 2-delta-delta Ct method [PMC7515963]. In a study comparing patients with mild cognitive impairment due to Alzheimer's disease (MCIAD) and patients with Alzheimer's disease (AD), it was found that the expression of hsa-mir3658 was upregulated in blood leukocytes as well as in cerebrospinal fluid (CSF) and serum from MCIAD patients compared to AD patients [PMC7515963]. Hsa-mir3658 was not found to be largely expressed in all examined tissues [PMC7515963]. The expression of these four microRNAs (including hsa-mir3658) was further analyzed in blood leukocytes as well as in CSF and serum from MCIAD patients [PMC7515963]. These findings were part of a study that analyzed the expression of selected microRNAs in various biological samples from MCIAD and AD-dementia patients according to IWG2 criteria [PMC7515963].