hsa-mir-608: Hsa-mir-608 is a primate-specific miRNA that has been identified and found to be induced by TCDD treatment in human neuroblastoma cells [PMC5578991]. To study the effects of hsa-mir-608, mimic and hairpin inhibitor transfected cells were used, and cell death was detected using the Annexin V-FITC detection kit [PMC3858247]. The study was conducted 72 hours after transfection with miRIDIAN microRNA human hsa-mir-608 mimics/hairpin inhibitors [PMC3858247]. The Annexin V-FITC detection kit is a commonly used method to detect cell death by measuring phosphatidylserine externalization, which is an early marker of apoptosis [PMC3858247]. The protocol for using the Annexin V-FITC detection kit was followed according to the manufacturer's instructions [PMC3858247]. This study provides evidence that hsa-mir-608 plays a role in cell death processes, potentially through its regulation of target genes involved in apoptosis pathways. Further research is needed to fully understand the mechanisms by which hsa-mir-608 influences cell death and its potential implications in neuroblastoma.

MIR608: MIR608 is a gene that encodes a small microRNA (miRNA) and is involved in regulating gene expression by altering the stability and translation of mRNAs [PMC5571768]. The rs4919510 variation in MIR608 has been found to influence miRNA activities by binding to MIR608 target sites within CD4 antigen, growth hormone receptor (GHR), retinoic X receptor beta (RXRB), and tumour protein p53 (TP53) genes with lower free energies than the wild type allele [PMC6046227]. This variation has also been associated with an increased risk of tuberculosis (TB) [PMC8924412]. In addition, the rs4919510 CC genotype of MIR608 has been found to be significantly over-represented in tendinopathy participants compared to asymptomatic controls, suggesting a potential role in tendon injury risk [PMC6006531]. However, the evidence for the role of MIR608 in altering tendon injury risk is inconclusive [PMC6628064]. Furthermore, variations in MIR608 have been studied in relation to Achilles tendinopathy, with some studies finding an association between certain polymorphisms and tendinopathy risk [PMC5436990] [PMC5571768] [PMC9147569]. Additionally, MIR608 has been shown to regulate the expression of BMP2 by sponging miR130a-3p, miR136-5p, mir18a-3p, and MIR608 itself [PMC8960627]. Overall, these studies suggest that variations in MIR608 may have an impact on gene regulation and disease susceptibility.